Regulatory
Part:BBa_K4779006:Design
Designed by: Ziqi Mi Group: iGEM23_Nanjing-BioXstem (2023-09-27)
UASprm1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
UASprm1 was obtained by PCR amplification using S.cerevisiae BY4741 genome as a template. The sequence is derived from -280bp to -101bp upstream of the pprm1 start codon.
References
John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895. Mark Rosenkrantz et al. Distinct upstream activation regions for glucose-repressed and derepressed expression of the yeast citrate synthase gene CIT1[J]. Current Genetics, 1994, 25(3) : 185-195.